质粒名称: | pMK-N. tabacum trnI/trnA |
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出品公司: | 爱迪基因 |
目录编号: | 123579 |
存储实验室: | Scott Lenaghan, C. Neal Stewart |
载体骨架基本信息 |
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载体名称: | pMK-RQ |
载体出品公司: | -- |
载体抗性: | 卡那霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 植物表达;叶绿体转化载体 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | N. tabacum trnI/trnA integration site |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pMK-N. tabacum trnI/trnA was a gift from Scott Lenaghan & C. Neal Stewart (Addgene plasmid # 123579 ; http://n2t.net/addgene:123579 ; RRID:Addgene_123579) |
参考文献部分: | MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S. Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. 10.1104/pp.18.01220 PubMed 30679266 |
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载体基本信息
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载体简介
载体序列
LOCUS pMK-N. tabacum trnI/trnA 5116 bp ds-DNA circular SYN 27-JUN-2019 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-N. tabacum trnI/trnA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5116) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 5116) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 27, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5116 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 1638..2357 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1683..1704) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1944..1963) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2291..2312 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(3322..3339) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3432..4020) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3512..3531) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4194..4988) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(4989..5093) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatg ggccttgtac acaccgcccg tcacactatg ggagctggcc atgcccgaag 421 tcgttacctt aaccgcaagg agggggatgc cgaaggcagg gctagtgact ggagtgaagt 481 cgtaacaagg tagccgtact ggaaggtgcg gctggatcac ctccttttca gggagagcta 541 atgcttgttg ggtattttgg tttgacactg cttcacaccc ccaaaaaaaa gaagggagct 601 acgtctgagt taaacttgga gatggaagtc atctttcctt tctcgacggt gaagtaagac 661 caagctcatg agcttattat cctaggtcgg aacaagttga taggaccccc ttttttacgt 721 ccccatgttc cccccgtgtg gcgacatggg ggcgaaaaaa ggaaagagag ggatggggtt 781 tctctcgctt ttggcatagc gggcccccag tgggaggctc gcacgacggg ctattagctc 841 agtggtagag cgcgcccctg ataattgcgt cgttgtgcct gggctgtgag ggctctcagc 901 cacatggata gttcaatgtg ctcatcggcg cctgaccctg agatgtggat catccaaggc 961 acattagcat ggcgtactcc tcctgttcga accggggttt gaaaccaaac tcctcctcag 1021 gaggatagat ggggcgattc gggtgagatc caatgtagat ccaactttcg attcactcgt 1081 gggatccggg cggtccgggg gggaccacca cggctcctct cttctcgaga atccatacat 1141 cccttatcag tgtatggaca gctatctctc gagcacaggt ttagcaatgg gaaaataaaa 1201 tggagcacct aacaacgcat cttcacagac caagaactac gagatcgccc ctttcattct 1261 ggggtgacgg agggatcgta ccattcgagc cgtttttttc ttgactcgaa atgggagcag 1321 gtttgaaaaa ggatcttaga gtgtctaggg ttgggccagg aggctctctt aacgccttct 1381 tttttcttct catcggagtt atttcacaaa gacttgccag ggtaaggaag aaggggggaa 1441 caagcacact tggagagcgc agtacaacgg agagttgtat gctgcgttcg ggaaggatga 1501 atcgctcccg aaaaggaatc tattgattct ctcccaattg gttggaccgt aggtgcgatg 1561 atttacttca cgggcgagct ctctggttca agtccaggat ggcccagctg cgaattcgtt 1621 taaactgcct tgtcttcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 1681 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 1741 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 1801 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct 1861 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 1921 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 1981 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 2041 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg 2101 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg 2161 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 2221 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga 2281 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 2341 acgagctgta caagtaagaa gacaagggag tttaaacaag cttctccact tggctcgggg 2401 ggatatagct cagttggtag agctccgctc ttgcaattgg gtcgttgcga ttacgggttg 2461 gatgtctaat tgtccaggcg gtaatgatag tatcttgtac ctgaaccggt ggctcacttt 2521 ttctaagtaa tggggaagag gaccgaaacg tgccactgaa agactctact gagacaaaga 2581 tgggctgtca agaacgtaga ggaggtagga tgggcagttg gtcagatcta gtatggatcg 2641 tacatggacg gtagttggag tcggcggctc tcccagggtt ccctcatctg agatctctgg 2701 ggaagaggat caagttggcc cttgcgaaca gcttgatgca ctatctccct tcaacccttt 2761 gagcgaaatg cggcaaaaga aaaggaagga aaatccatgg accgacccca tcatctccac 2821 cccgtaggaa ctacgagatc accccaagga cgccttcggc atccaggggt cacggaccga 2881 ccatagaacc ctgttcaata agtggaacgc attagctgtc cgctctcagg ttgggcagtc 2941 agggtcggag aagggcaatg actcattctt agttagaatg ggattccaac tcagcacctt 3001 ttgagtgaga ttttgagaag agttgctctt tggagagcac agtacgatga aagttgtaag 3061 ctgtgttcgg gggggagtta ttgtctatcg ttggcctcta tggtagaatc agtcggggga 3121 cctgagaggc ggtggtttac cctgcggcgg atgtcagcgg ttcgagtccg cttatctcca 3181 actcgtgaac ttagccgata caaagctctg ggcctcatgg gccttccttt cactgcccgc 3241 tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct gtttccttgc 3301 gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa 3361 gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 3421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 3481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 3541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 3601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 3661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 3721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 3901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 4021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 4081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 4141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttattagaaa 4201 aattcatcca gcagacgata aaacgcaata cgctggctat ccggtgccgc aatgccatac 4261 agcaccagaa aacgatccgc ccattcgccg cccagttctt ccgcaatatc acgggtggcc 4321 agcgcaatat cctgataacg atccgccacg cccagacggc cgcaatcaat aaagccgcta 4381 aaacggccat tttccaccat aatgttcggc aggcacgcat caccatgggt caccaccaga 4441 tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca gctctgccgg tgccaggccc 4501 tgatgttctt catccagatc atcctgatcc accaggcccg cttccatacg ggtacgcgca 4561 cgttcaatac gatgtttcgc ctgatgatca aacggacagg tcgccgggtc cagggtatgc 4621 agacgacgca tggcatccgc cataatgctc actttttctg ccggcgccag atggctagac 4681 agcagatcct gacccggcac ttcgcccagc agcagccaat cacggcccgc ttcggtcacc 4741 acatccagca ccgccgcaca cggaacaccg gtggtggcca gccagctcag acgcgccgct 4801 tcatcctgca gctcgttcag cgcaccgctc agatcggttt tcacaaacag caccggacga 4861 ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc caatggtctg ctgcgcccaa 4921 tcatagccaa acagacgttc cacccacgct gccgggctac ccgcatgcag gccatcctgt 4981 tcaatcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5101 ccccgaaaag tgccac //